1JYN
E. COLI (lacZ) BETA-GALACTOSIDASE (E537Q) IN COMPLEX WITH LACTOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 149.550, 168.630, 200.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.800 |
| Rwork | 0.155 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.938 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.190 |
| Number of reflections | 433670 | |
| <I/σ(I)> | 12.5 | 4.1 |
| Completeness [%] | 92.0 | 81.9 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 288 | used macroseeding, Juers, D.H., (2000) Protein Sci., 9, 1685. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 10 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 2 | 1 | drop | Bis-Tris | 100 (mM) | pH6.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | reservoir | PEG8000 | 10 (%) | |
| 7 | 1 | reservoir | bis-Tris | 100 (mM) | pH6.5 |
| 8 | 1 | reservoir | 200 (mM) | ||
| 9 | 1 | reservoir | 100 (mM) |
