1JYD
Crystal Structure of Recombinant Human Serum Retinol-Binding Protein at 1.7 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | H 3 |
| Unit cell lengths | 101.336, 101.336, 71.978 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 * - 1.700 |
| R-factor | 0.251 |
| Rwork | 0.248 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rbp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.469 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.047 * | 0.505 * |
| Total number of observations | 67359 * | |
| Number of reflections | 28189 | |
| <I/σ(I)> | 7.68 | 2.59 |
| Completeness [%] | 90.5 | 91 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 | 16 * | Sodium cacodylate, sodium chloride, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 3 | 1 | drop | 3.9 (M) | pH6.8 | |
| 4 | 1 | reservoir | sodium cacodylate | 20 (mM) | |
| 5 | 1 | reservoir | 4.5 (M) | pH6.8 |
