1JXP
BK STRAIN HEPATITIS C VIRUS (HCV) NS3-NS4A
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 96.960, 96.960, 167.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 * - 2.200 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.35200 * |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.310 * |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | |
| Number of reflections | 22953 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 94.0 | 72 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 4 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protease | 22 (mg/ml) | |
| 2 | 1 | drop | glycerol | 10 (%) | |
| 3 | 1 | drop | dithiothreitol | 5 (mM) | |
| 4 | 1 | drop | HEPES | 25 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | sodium cacodylate | 0.1 (M) |
