1JXN
Crystal Structure of the Lectin I from Ulex europaeus in complex with the methyl glycoside of alpha-L-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-14 |
| Detector | BRANDEIS |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.810, 69.000, 119.020 |
| Unit cell angles | 90.00, 106.76, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.2021 |
| Rwork | 0.202 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fx5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.670 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.000 | 2.300 |
| Number of reflections | 44883 | |
| <I/σ(I)> | 14.93 | 2.62 |
| Completeness [%] | 87.9 | 76.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 287 | MPD, PEG 550MME, sodium acetate, sodium cacodylate, calcium chloride, manganese chloride, pH 4.0, VAPOR DIFFUSION, HANGING DROP at 287K |
