1JXB

I53A, a point mutant of the cysteine-free variant of E. coli Rnase HI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-19
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	36.867, 40.830, 85.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.500 - 1.600
R-factor	0.207 *
Rwork	0.207
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.062 *
Number of reflections	34997
Completeness [%]	95.2	91.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	20 (mM)	pH7.5
2	1	drop	PEG3350	10 (%)
3	1	drop	protein	4 (mg/ml)