1JWQ
Structure of the catalytic domain of CwlV, N-acetylmuramoyl-L-alanine amidase from Bacillus(Paenibacillus) polymyxa var.colistinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.708 |
| Spacegroup name | P 61 |
| Unit cell lengths | 66.500, 66.500, 88.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.176 |
| R-free | 0.20600 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.940 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.092 | 0.198 |
| Number of reflections | 34876 | |
| Completeness [%] | 97.1 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG4000, lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
