1JWO
Crystal Structure Analysis of the SH2 Domain of the Csk Homologous Kinase CHK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.146, 58.696, 28.817 |
| Unit cell angles | 90.00, 115.36, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.19425 |
| Rwork | 0.191 |
| R-free | 0.25679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sha |
| RMSD bond length | 0.046 |
| RMSD bond angle | 4.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.108 | 0.304 |
| Number of reflections | 3237 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 94.3 | 80.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 292 | AMMONIUM SULFATE, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 292.0K |
