1JWG

VHS Domain of human GGA1 complexed with cation-independent M6PR C-terminal Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-16
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	56.146, 65.770, 101.426
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.22796
Rwork	0.226
R-free	0.26115
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jwf
RMSD bond length	0.011
RMSD bond angle	1.286
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.067 *
Total number of observations	167862 *
Number of reflections	25975 *
Completeness [%]	99.6	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	293	PEG 3350, ammonium iodide, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	1 (mM)	pH8.0
2	1	drop	protein	10 (mg/ml)
3	1	reservoir	PEG3350	14 (%(w/v))
4	1	reservoir		0.2 (M)
5	1	reservoir	Tris-HCl	0.1 (M)	pH7.5