1JWB
Structure of the Covalent Acyl-Adenylate Form of the MoeB-MoaD Protein Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-08-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 77.230, 77.230, 100.545 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.188 * |
Rwork | 0.188 |
R-free | 0.22494 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.020 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.086 | 0.414 |
Number of reflections | 18549 | |
<I/σ(I)> | 8.6 | 1.3 |
Completeness [%] | 98.7 | 98.1 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | Lithium Sulfate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | MoeB | 23 (mg/ml) | |
2 | 1 | drop | MoaD | 10 (mg/ml) | |
3 | 1 | reservoir | 1.7 (M) | ||
4 | 1 | reservoir | HEPES | 100 (mM) |