1JW4

Structure of ligand-free maltodextrin-binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	1999-11-08
Detector	MACSCIENCE
Spacegroup name	P 1
Unit cell lengths	38.100, 44.600, 58.300
Unit cell angles	99.60, 101.60, 104.50

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.2 *
Rwork	0.200
R-free	0.26000
RMSD bond length	0.010 *
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.970
High resolution limit [Å]	2.000 *	1.900
Rmerge	0.043	0.165
Number of reflections	24467
<I/σ(I)>	16
Completeness [%]	87.7	57.9
Redundancy	1.6 *	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6 *	298	PEG 20k, Mes, sodium azide, pH 6.2, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MBP	3.5 (mg/ml)
2	1	drop	maltotetraitol	1 (mM)
3	1	drop	PEG3350	20 (%)	pH6.6
4	1	reservoir	PEG3350	30 (%)