1JVM
KCSA POTASSIUM CHANNEL WITH TBA (TETRABUTYLAMMONIUM) AND RUBIDIUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.800, 69.400, 112.700 |
| Unit cell angles | 90.00, 125.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.281 * |
| Rwork | 0.281 |
| R-free | 0.30210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bl8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.394 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.048 | 0.088 |
| Number of reflections | 14799 | |
| <I/σ(I)> | 21 | |
| Completeness [%] | 0.7 | 0.167 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 20 * | PEG400,calcium chloride, Hepes, potassium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
| 2 | 1 | reservoir | 120-300 (mM) | ||
| 3 | 1 | reservoir | PEG400 | 35-50 (%) | |
| 4 | 1 | drop | LDAO | 5 (mM) | |
| 5 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 6 | 1 | drop | dithiothreitol | 2 (mM) | |
| 7 | 1 | drop | TBA | 5 (mM) |
