1JVL

Azurin dimer, covalently crosslinked through bis-maleimidomethylether

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-09
Detector	MARRESEARCH
Wavelength(s)	1.0500
Spacegroup name	P 61
Unit cell lengths	48.605, 48.605, 284.809
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.000
Rwork	0.190
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.070 *	0.440 *
Number of reflections	25535
<I/σ(I)>	15	2.1
Completeness [%]	99.5	99.9
Redundancy	3.2	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9 *	293	PEG 2000 MME, nickel chloride, Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG2000 MME	20 (%(w/v))
3	1	reservoir		0.01 (M)
4	1	reservoir	Tris-HCl	0.1 (M)	pH9.0