1JVK

THREE-DIMENSIONAL STRUCTURE OF AN IMMUNOGLOBULIN LIGHT CHAIN DIMER ACTING AS A LETHAL AMYLOID PRECURSOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-05-08
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	48.280, 83.320, 112.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 1.940
R-factor	0.223 *
Rwork	0.221
R-free	0.25400 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010 *
RMSD bond angle	1.730 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.010
High resolution limit [Å]	1.940	1.940
Rmerge	0.043 *	0.319
<I/σ(I)>	29.6	3.78
Completeness [%]	98.2 *	89.1
Redundancy	4. *

Crystallization Conditions

crystal ID	method	pH	temperature	details
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