1JUL

INDOLE-3-GLYCEROLPHOSPHATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS IN A SECOND ORTHORHOMBIC CRYSTAL FORM

Experimental procedure

Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-01-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	62.600, 74.000, 74.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.000
R-factor	0.176
Rwork	0.176
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1igs
RMSD bond length	0.009
RMSD bond angle	1.920
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.400	2.060
High resolution limit [Å]	1.900	2.000
Rmerge	0.078	0.256
Number of reflections	27656
<I/σ(I)>	7.8	2.9
Completeness [%]	99.5	100
Redundancy	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	4 *	pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	10 (%(w/v))
2	1	reservoir	etylene glycol	8 (%(v/v))
3	1	reservoir	MES	0.1 (M)