1JUG
LYSOZYME FROM ECHIDNA MILK (TACHYGLOSSUS ACULEATUS)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.120, 41.980, 38.090 |
| Unit cell angles | 90.00, 91.04, 90.00 |
Refinement procedure
| Resolution | 7.000 - 1.900 |
| R-factor | 0.17 |
| Rwork | 0.168 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lyz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.042 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 * | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | |
| Number of reflections | 8418 | |
| Completeness [%] | 90.0 | 75.7 * |
| Redundancy | 4.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * | 1:1 mixture of drop solution and well solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | citrate | 10 (mM) | |
| 3 | 1 | reservoir | 0.05 (M) | ||
| 4 | 1 | reservoir | PEG8000 | 20 (%) |
