1JUD
L-2-HALOACID DEHALOGENASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-07 |
| Detector | RIGAKU |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.210, 62.780, 50.840 |
| Unit cell angles | 90.00, 122.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.330 * |
| Data reduction software | R-AXIS (IIC) |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.040 | 0.109 |
| Total number of observations | 31020 * | |
| Number of reflections | 8124 | |
| Completeness [%] | 93.0 | 90.3 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 4 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme solution | 15 (mg/ml) | |
| 2 | 1 | reservoir | potassium dihydrogenphosphate | 50 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
| 4 | 1 | reservoir | n-propanol alcohol | 1 (%(v/v)) |
