1JUC
Crystal Structure Analysis of a Holliday Junction Formed by CCGGTACCGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.073 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.871, 25.364, 37.373 |
| Unit cell angles | 90.00, 110.57, 90.00 |
Refinement procedure
| Resolution | 34.920 - 2.350 |
| R-factor | 0.22841 |
| Rwork | 0.226 |
| R-free | 0.26779 |
| RMSD bond length | 0.067 |
| RMSD bond angle | 2.195 |
| Data reduction software | XFIT |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.056 * | 0.230 * |
| Total number of observations | 4466 * | |
| Number of reflections | 2390 * | |
| Completeness [%] | 96.4 * | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 290 | 40 mM sodium cacodylate (pH 7.0), 12 mM spermine, 80 mM KCl, 10% (v/v) MPD equilibrated against 35% MPD with oligo concentration of 1 mM, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 290.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium cacodylate | ||
| 2 | 1 | 1 | spermine | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 40 (mM) | pH7.0 | |
| 2 | 1 | drop | 12 (mM) | ||
| 4 | 1 | drop | 80 (mM) | ||
| 5 | 1 | drop | 10 (%(v/v)) | ||
| 6 | 1 | drop | d(CCGGTACCGG)2 | 1 (mM) | |
| 7 | 1 | reservoir | MPD | 35 (%) |
