1JTO

Degenerate interfaces in antigen-antibody complexes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	298
Detector technology	DIFFRACTOMETER
Collection date	1996-05-01
Detector	ENRAF-NONIUS FAST
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	68.680, 69.710, 113.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.173 *
Rwork	0.173
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mel
Data reduction software	ENRAF (NONIUS)
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.114	0.420
Number of reflections	17829
<I/σ(I)>	6.1	1.8
Completeness [%]	93.4	84.8
Redundancy	5.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.6 *	300	PEG 8000, potassium phosphate, , pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	2 (M)
2	1	reservoir	sodium citrate	0.1 (M)