1JTC
Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form with Amino Terminal His Tag AND LEU 44 REPLACED BY PHE (L44F)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2000-07-10 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.923, 73.784, 109.131 |
Unit cell angles | 90.00, 89.98, 90.00 |
Refinement procedure
Resolution | 36.900 - 1.700 |
R-factor | 0.193 * |
R-free | 0.23900 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jqz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MRCHK |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.900 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.046 | 0.319 |
Total number of observations | 274969 * | |
Number of reflections | 72647 * | |
<I/σ(I)> | 26.5 | 2.6 |
Completeness [%] | 88.1 * | 64.8 * |
Redundancy | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 or 10 * | sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9-12 (mg/ml) | |
2 | 1 | reservoir | sodium formate | 3.4-4.4 (M) | |
3 | 1 | reservoir | ammonium sulfate | 1.0-1.2 (M) |