1JT9

Structure of the mutant F174A T form of the Glucosamine-6-Phosphate deaminase from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2000-01-15
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 63 2 2
Unit cell lengths	127.617, 127.617, 139.822
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.130 - 2.060
R-factor	0.204 *
Rwork	0.204
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cd5
RMSD bond length	0.009
RMSD bond angle	2.700
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.020	2.060
Rmerge	0.050 *	0.334 *
Number of reflections	41650 *
<I/σ(I)>	11.1	2.3
Completeness [%]	99.0
Redundancy	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	18 *	HEPES, sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	2.9 (M)
2	1	reservoir	HEPES	100 (mM)	pH7.0
3	1	reservoir	GlcNAc6P	5 (mM)