1JT4

Human Acidic Fibroblast Growth Factor. 141 Amino Acid Form with Amino Terminal His Tag AND VAL 109 REPLACED BY LEU (V109L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2000-04-26
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	73.556, 97.356, 109.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.900 - 1.780
R-factor	0.198 *
R-free	0.24500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jqz
RMSD bond length	0.009
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MRCHK
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.900	1.820
High resolution limit [Å]	1.780	1.780
Rmerge	0.063 *	0.372
Total number of observations	315069 *
Number of reflections	35538 *
<I/σ(I)>	31.4	3.4
Completeness [%]	95.4 *	85.1 *
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	4 or 10 *	sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9-12 (mg/ml)
2	1	reservoir	sodium formate	3.4-4.4 (M)
3	1	reservoir	ammonium sulfate	1.0-1.2 (M)