1JSW

NATIVE L-ASPARTATE AMMONIA LYASE

Experimental procedure

Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1996-04-26
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 2
Unit cell lengths	153.500, 146.200, 103.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800 *
R-factor	0.216
Rwork	0.216
R-free	0.37100
RMSD bond length	0.010
RMSD bond angle	22.400 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.076
Total number of observations	156675 *
Number of reflections	57964
Completeness [%]	80.3
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.5 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10-13 (mg/ml)
2	1	1	Tris-HEPES	50 (mM)
3	1	2	Tris-HCl	0.1 (M)
4	1	2	PEG3350	26 (%)
5	1	2	sodium acetate	0.30 (M)
6	1	2	dithiothreitol	4 (mM)