1JSD

CRYSTAL STRUCTURE OF SWINE H9 HAEMAGGLUTININ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	108.500, 108.500, 149.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.218
Rwork	0.218
R-free	0.23000
Structure solution method	MIR
RMSD bond length	0.007 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.083 *	0.482 *
Number of reflections	91194	9090 *
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	na citrate, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	reservoir	sodium citrate	1.4 (M)
4	1	reservoir	HEPES	100 (mM)