1JRS
HEMIACETAL COMPLEX BETWEEN LEUPEPTIN AND TRYPSIN
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-19 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.686, 69.373, 63.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.20700 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.600 |
| Data reduction software | PROCESS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.044 |
| Number of reflections | 78707 |
| Completeness [%] | 88.8 |
| Redundancy | 3.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | ORTHORHOMBIC SPACE GROUP; CRYSTALLIZATION CONDITIONS: 24-26% PEG8000, 0.02M INHIBITOR, 0.2M (NH4)2 SO4, 50MM TRIS-HCL, ROOM TEMPERATURE, HANGING DROP., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30-40 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 24-26 (%) | |
| 3 | 1 | reservoir | inhibitor | 0.02 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) |
