1JRI
The Crystal Structure of an Sm-like Archaeal Protein with Two Heptamers in the Asymmetric Unit.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-04-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.371, 114.698, 238.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.950 - 2.800 |
| R-factor | 0.199 * |
| Rwork | 0.199 |
| R-free | 0.29000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jbm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.440 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.112 | 0.387 |
| Total number of observations | 329838 * | |
| Number of reflections | 28487 | |
| <I/σ(I)> | 19.3 | 3.3 |
| Completeness [%] | 99.0 | 92.5 |
| Redundancy | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 292.8 | 0.1 M Tris, 10% v/v isopropanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 19.8K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 42 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.8 |
| 3 | 1 | drop | EDTA | 5 (mM) | pH8.0 |
| 4 | 1 | drop | 0.1 (M) | ||
| 5 | 1 | reservoir | Tris | 0.1 (M) | pH8.50 |
| 6 | 1 | reservoir | isopropanol | 10 (%(v/v)) |
