1JRB
The P56A mutant of Lactococcus lactis dihydroorotate dehydrogenase A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.992 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.074, 108.822, 66.453 |
| Unit cell angles | 90.00, 103.92, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.196 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Lactococcus lactis DHODA PDB ID 2DOR |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.035 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.046 | 0.187 |
| Number of reflections | 57637 | |
| <I/σ(I)> | 29.9 | 6.2 |
| Completeness [%] | 93.6 | 82.2 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 6K, Na-acetate, TRIS-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
