1JQO
Crystal structure of C4-form phosphoenolpyruvate carboxylase from maize
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-25 |
| Detector | FUJI |
| Wavelength(s) | 0.708 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 158.440, 174.610, 254.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.200 - 3.000 |
| Rwork | 0.236 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 20.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.200 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.091 | 0.309 |
| Total number of observations | 266008 * | |
| Number of reflections | 58078 | |
| Completeness [%] | 80.4 | 57.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | 293 | PEG8000, Tris-HCl, LiSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | ethylene glycol | 20 (%) | |
| 5 | 1 | drop | malate | 5 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 12 (%) | |
| 7 | 1 | reservoir | 400 (mM) | ||
| 8 | 1 | reservoir | ethylene glycol | 20 (%) |
