1JQH
IGF-1 receptor kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.050 |
| Spacegroup name | P 32 |
| Unit cell lengths | 85.963, 85.963, 132.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.100 |
| R-factor | 0.195 * |
| Rwork | 0.195 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNX (2000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.047 | 0.440 |
| Total number of observations | 160185 * | |
| Number of reflections | 63650 | |
| <I/σ(I)> | 16.9 | 2.3 |
| Completeness [%] | 99.3 | 98.7 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | magnesium chloride, Tris, ammonium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | AMP-PNP | 2 (mM) | |
| 2 | 1 | reservoir | PEG5000 MME | 26-30 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | pH7.0 |
