1JQA
Bacillus stearothermophilus glycerol dehydrogenase complex with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 1999-11-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.970 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 105.910, 105.910, 149.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.050 |
| Rwork | 0.158 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jpu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.465 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.081 | 0.288 |
| Number of reflections | 25033 | |
| <I/σ(I)> | 13.2 | 2.9 |
| Completeness [%] | 93.5 | 72 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | Ammonium Sulfate, PEG 400, ZnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
