1JPR
Mn substituted Ribonucleotide reductase R2 from E. coli oxidized by nitric oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.880 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.826, 84.683, 114.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.880 |
| R-factor | 0.158 * |
| Rwork | 0.158 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Reduced wt E. coli R2 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.790 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.950 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.069 | 0.273 |
| Number of reflections | 57618 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 20 * | Nordlund, P., (1989) FEB Lett., 258, 251. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 20 (%) | |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | reservoir | dioxane | 0.3 (%) | |
| 4 | 1 | reservoir | MES | 0.05 (M) | |
| 5 | 1 | drop | protein | 20 (mg/ml) |
