1JPN
GMPPNP Complex of SRP GTPase NG Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.815, 54.531, 99.078 |
| Unit cell angles | 90.00, 97.42, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.19 * |
| Rwork | 0.190 |
| R-free | 0.24000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ffh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.946 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.051 | 0.076 |
| Number of reflections | 45606 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 4 * | 29% MPD, 100 mM NaAc, 20mM CaCl2, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-30 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | reservoir | MPD | 29 (%) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 5 | 1 | reservoir | 20 (mM) |
