1JPC
MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM SNOWDROP (GALANTHUS NIVALIS) BULBS IN COMPLEX WITH MANNOSE-ALPHA1,6-(MANNOSE-ALPHA1,3)-MANNOSE-ALPHA1,6-(MANNOSE-ALPHA1,3)-MANNOSE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-07 |
| Detector | RIGAKU |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 96.339, 96.339, 68.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22000 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.360 * |
| Data reduction software | MOSFLM (5.23) |
| Data scaling software | SCALA |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.000 * | |
| Rmerge | 0.074 | |
| Total number of observations | 54560 * | |
| Number of reflections | 11166 | |
| Completeness [%] | 99.7 | 99.6 * |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium salfate | 33 (%sat) | |
| 2 | 1 | reservoir | ammonium bicarbonate | 0.05 (M) | |
| 3 | 1 | drop | protein | 3.5 (mg/ml) | |
| 4 | 1 | drop | Man-5 | 0.28 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 9 (%) |
