1JOP
YHCH protein (HI0227)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0053, 1.0087 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.100, 152.300, 53.400 |
| Unit cell angles | 90.00, 113.60, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.047 | 0.131 |
| Number of reflections | 19314 | |
| <I/σ(I)> | 19.9 | 6.2 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Na acetate, PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
