1JOG
Structure of HI0074 from Heamophilus Influenzae reveals the fold of a substrate binding domain of a nucleotidyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9791, 0.9793, 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.300, 87.100, 165.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| Rwork | 0.211 |
| R-free | 0.28200 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.067 | 0.314 |
| Number of reflections | 22467 | |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 294 | 30% PEG 4000, 0.1 M Tris, 0.2 M Na acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.7 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 30 (%) | |
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
| 8 | 1 | reservoir | sodium acetate | 0.2 (M) |
