1JND
Crystal structure of imaginal disc growth factor-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9464 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 106.345, 106.345, 89.987 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 1.300 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ctn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.330 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.023 * | 0.257 * |
| Total number of observations | 758311 * | |
| Number of reflections | 129904 | |
| <I/σ(I)> | 2.88 | |
| Completeness [%] | 90.3 | 53.5 |
| Redundancy | 1.1 | 1.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5 | 4 * | PEG 4000, sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 2 | PEG4000 | 12 (%) | |
| 3 | 1 | 2 | sodium acetate | 0.1 (M) | pH5.0 |
