1JNB

CONNECTOR PROTEIN FROM BACTERIOPHAGE PHI29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-08-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	177.160, 169.240, 185.440
Unit cell angles	90.00, 114.10, 90.00

Refinement procedure

Resolution	9.000 - 3.200
R-factor	0.2844 *
Rwork	0.284
R-free	0.30140
Structure solution method	molecular replacement and MIR
Starting model (for MR)	low resolution model based on cryo-EM data
RMSD bond length	0.001
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE (+ DM)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.240
High resolution limit [Å]	3.200 *	3.210
Rmerge	0.065 *	0.240 *
Number of reflections	78035
<I/σ(I)>	21
Completeness [%]	99.5	99 *
Redundancy	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8	292	30-40% MPD, 0.1 M Tris-HCl pH 8.0, 0.05 M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	MPD	36-40 (%)
2	1	1		0.05 (M)
3	1	1	Tris-HCl	0.1 (M)