1JNB
CONNECTOR PROTEIN FROM BACTERIOPHAGE PHI29
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-08-14 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.160, 169.240, 185.440 |
Unit cell angles | 90.00, 114.10, 90.00 |
Refinement procedure
Resolution | 9.000 - 3.200 |
R-factor | 0.2844 * |
Rwork | 0.284 |
R-free | 0.30140 |
Structure solution method | molecular replacement and MIR |
Starting model (for MR) | low resolution model based on cryo-EM data |
RMSD bond length | 0.001 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE (+ DM) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.240 |
High resolution limit [Å] | 3.200 * | 3.210 |
Rmerge | 0.065 * | 0.240 * |
Number of reflections | 78035 | |
<I/σ(I)> | 21 | |
Completeness [%] | 99.5 | 99 * |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8 | 292 | 30-40% MPD, 0.1 M Tris-HCl pH 8.0, 0.05 M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | MPD | 36-40 (%) | |
2 | 1 | 1 | 0.05 (M) | ||
3 | 1 | 1 | Tris-HCl | 0.1 (M) |