1JN5

Structural basis for the recognition of a nucleoporin FG-repeat by the NTF2-like domain of TAP-p15 mRNA export factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-03
Detector	MARRESEARCH
Wavelength(s)	0.933
Spacegroup name	P 65 2 2
Unit cell lengths	105.200, 105.200, 172.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.241 *
Rwork	0.241
R-free	0.26700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1jkg
RMSD bond length	0.008
RMSD bond angle	1.426
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	30.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.054	0.351
Total number of observations	233306 *
Number of reflections	14630 *
Completeness [%]	98.1	99.9
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5 *	18 *	Na/K Tartrate, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	drop		50 (mM)
4	1	drop	dithiothreitol	0.5 (mM)
5	1	reservoir	sodium potassium tartrate	0.8 (M)
6	1	reservoir	MES	100 (mM)