1JN3
FIDELITY PROPERTIES AND STRUCTURE OF M282L MUTATOR MUTANT OF DNA POLYMERASE: SUBTLE STRUCTURAL CHANGES INFLUENCE THE MECHANISM OF NUCLEOTIDE DISCRIMINATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-10 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.540, 63.620, 37.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.480 - 2.350 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.25600 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.480 | 2.530 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.080 | 0.244 * |
| Total number of observations | 89772 * | |
| Number of reflections | 11677 * | |
| Completeness [%] | 93.4 | 65.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | 16% PEG 3350, 5mM ammonium sulfate, 150mM sodium acetate, 50mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 10 (mM) | |
| 3 | 1 | drop | MES | 0.1 (M) | |
| 4 | 1 | reservoir | PEG3350 | 13-20.0 (%) | |
| 5 | 1 | reservoir | sodium acetate | 50-300 (mM) | |
| 6 | 1 | reservoir | HEPES | 50 (mM) |
