1JMV

Structure of Haemophylus influenzae Universal Stress Protein At 1.85A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-12-18
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 21 21 21
Unit cell lengths	64.129, 63.214, 136.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.780 - 1.850
R-factor	0.22 *
Rwork	0.220
R-free	0.24200
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.910
High resolution limit [Å]	1.850	1.850
Rmerge	0.041	0.103
Total number of observations	339160 *
Number of reflections	49785
<I/σ(I)>	29.4	14.4
Completeness [%]	99.4	96.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 400, sodium cacodylate, ammonium sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	PEG4000	21 (%)
3	1	reservoir	ammonium sulfate	200 (mM)
4	1	reservoir	sodium cacodylate	100 (mM)	pH6.5