1JMT

X-ray Structure of a Core U2AF65/U2AF35 Heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790
Spacegroup name	P 2 2 2
Unit cell lengths	41.520, 49.740, 92.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.360 - 2.200
R-factor	0.226
Rwork	0.226
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.340	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.042	0.254 *
Total number of observations	40388 *
Number of reflections	9935
<I/σ(I)>	21
Completeness [%]	97.4	98.3 *
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5 *	4 *	PEG mme5000, sodium acetate, 1,6-hexanediol, MES, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	5.5 *	4 *	PEG mme5000, sodium acetate, 1,6-hexanediol, MES, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	500 (mM)
2	1	reservoir	MES	100 (mM)
3	1	reservoir	PEG5000 MME	15 (%(w/v))
4	1	reservoir	1,6-hexanediol	5 (%(w/v))
5	1	reservoir	TCEP	0.5 (mM)
6	1	drop	protein	45 (mg/ml)