1JMS

Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	110
Detector technology	CCD
Collection date	1999-04-25
Detector	MARRESEARCH
Wavelength(s)	0.940
Spacegroup name	P 21 21 21
Unit cell lengths	47.100, 85.200, 111.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 2.350 *
R-factor	0.21 *
Rwork	0.214
R-free	0.25800 *
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	1.080 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.400
High resolution limit [Å]	2.350	2.350
Rmerge	0.059	0.340
Total number of observations	99454 *
Number of reflections	19351	1224 *
<I/σ(I)>	25.3	4.1
Completeness [%]	99.8	98.4
Redundancy	5.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8 *	253 *	Sukumar, N., (2000) Acta Crystallogr., Sect.D, 56, 1662. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH6.8
2	1	drop		200 (mM)
3	1	drop		50 (mM)
4	1	drop	ammonium sulfate	100 (mM)
5	1	drop	protein	10 (mg/ml)