1JMS
Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.940 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.100, 85.200, 111.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.350 * |
| R-factor | 0.21 * |
| Rwork | 0.214 |
| R-free | 0.25800 * |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.080 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.059 | 0.340 |
| Total number of observations | 99454 * | |
| Number of reflections | 19351 | 1224 * |
| <I/σ(I)> | 25.3 | 4.1 |
| Completeness [%] | 99.8 | 98.4 |
| Redundancy | 5.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 253 * | Sukumar, N., (2000) Acta Crystallogr., Sect.D, 56, 1662. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH6.8 |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | ammonium sulfate | 100 (mM) | |
| 5 | 1 | drop | protein | 10 (mg/ml) |
