1JLV

Anopheles dirus species B glutathione S-transferases 1-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2000-06-28
Detector	MARRESEARCH
Wavelength(s)	1.5405
Spacegroup name	P 43
Unit cell lengths	87.810, 87.810, 166.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.220 - 1.750
R-factor	0.223
Rwork	0.223
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Lucilia cuprina GST
RMSD bond length	0.006
RMSD bond angle	20.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	96781
<I/σ(I)>	9.4	2.2
Completeness [%]	77.0	85.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6 *	298	PEG 4000, glutathione, dithiothreitol, acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.2 (M)
2	1	reservoir	sodium acetate	0.1 (M)	pH4.6
3	1	reservoir	PEG4000	30 (%)