1JLJ
1.6 Angstrom crystal structure of the human neuroreceptor anchoring and molybdenum cofactor biosynthesis protein gephyrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.269, 66.008, 73.122 |
| Unit cell angles | 90.00, 93.27, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.14816 |
| Rwork | 0.147 |
| R-free | 0.18279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1di6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.399 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.116 * | 0.283 * |
| Number of reflections | 64743 | |
| <I/σ(I)> | 14.4 | 1.9 |
| Completeness [%] | 90.9 | 46.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 294 | sodium formate, ammonium, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 294K |
| 1 | Vapor diffusion * | 4.6 | 294 | sodium formate, ammonium, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 23 (mg/ml) | |
| 2 | 1 | reservoir | sodium formate | 0.6-0.7 (M) | |
| 3 | 1 | reservoir | acetate | 0.1 (M) |
