1JLJ

1.6 Angstrom crystal structure of the human neuroreceptor anchoring and molybdenum cofactor biosynthesis protein gephyrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-06
Detector	ADSC QUANTUM 4
Wavelength(s)	1.100
Spacegroup name	C 1 2 1
Unit cell lengths	114.269, 66.008, 73.122
Unit cell angles	90.00, 93.27, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.14816
Rwork	0.147
R-free	0.18279
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1di6
RMSD bond length	0.013
RMSD bond angle	1.399
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.116 *	0.283 *
Number of reflections	64743
<I/σ(I)>	14.4	1.9
Completeness [%]	90.9	46.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6	294	sodium formate, ammonium, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 294K
1	Vapor diffusion *	4.6	294	sodium formate, ammonium, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	23 (mg/ml)
2	1	reservoir	sodium formate	0.6-0.7 (M)
3	1	reservoir	acetate	0.1 (M)