1JL6
Crystal Structure of CN-Ligated Component IV Glycera Dibranchiata Monomeric Hemoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-05 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.011, 32.890, 40.710 |
| Unit cell angles | 90.00, 109.07, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.19 |
| Rwork | 0.179 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.231 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.053 | 0.135 |
| Number of reflections | 10950 | |
| Completeness [%] | 88.9 | 65 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | PEG 4000, sodium acetate, ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
