1JKW

STRUCTURE OF CYCLIN MCS2

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE
Synchrotron site	LURE
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-05-20
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	84.060, 84.060, 373.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.000 - 2.600
R-factor	0.222
Rwork	0.222
R-free	0.27200
RMSD bond length	0.015
RMSD bond angle	1.860
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.600 *
Rmerge	0.059
Total number of observations	21133 *
Number of reflections	20809
Completeness [%]	98.5
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium phosphate	1 (M)
2	1	reservoir	sodium citrate	0.1 (M)
3	1	reservoir	glycerol	2 (%)
4	1	reservoir	beta-ME	10 (mM)