1JKT
TETRAGONAL CRYSTAL FORM OF A CATALYTIC DOMAIN OF DEATH-ASSOCIATED PROTEIN KINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 58.370, 58.370, 212.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.720 - 3.500 |
| R-factor | 0.273 * |
| Rwork | 0.272 |
| R-free | 0.29700 * |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.690 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.000 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.072 | 0.390 |
| Number of reflections | 8704 | 892 * |
| Completeness [%] | 96.5 | 96.1 * |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (M) | pH7.5 |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | 0.1 (M) | ||
| 6 | 1 | reservoir | PEG1000 | 5 (%(w/v)) | |
| 7 | 1 | reservoir | PEG300 | 40 (%(v/v)) | |
| 8 | 1 | reservoir | Tris-HCl | 0.1 | pH7.0 |
