1JK6

UNCOMPLEXED DES 1-6 BOVINE NEUROPHYSIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-09-01
Detector	RIGAKU RAXIS IV
Spacegroup name	P 32
Unit cell lengths	48.920, 48.920, 78.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.000 - 2.400
R-factor	0.218
Rwork	0.218
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bn2
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	999.000
High resolution limit [Å]	2.300
Rmerge	0.042
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	2M SODIUM CHLORIDE, 0.1M MES BUFFER AND 0.05M SODIUM/POTASSIUM PHOSPATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K