1JJT
IMP-1 METALLO BETA-LACTAMASE FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH A BIARYL SUCCINIC ACID INHIBITOR (1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-03-27 |
| Detector | ADSC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.800, 45.180, 64.510 |
| Unit cell angles | 90.00, 100.32, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.199 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMEN |
| Starting model (for MR) | SAME PROTEIN IN A DIFFERENT SPACE GROUP |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.023 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | MERLOT |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9999.000 * | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | 0.274 |
| Number of reflections | 37199 | 5552 * |
| <I/σ(I)> | 10.838 | 1.417 |
| Completeness [%] | 98.0 | 89 |
| Redundancy | 3.315 | 2.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 310 | peg monomethyl ether 550, zinc acetate, mes pH 6.7-7.1, 310 K, pH 6.9, VAPOR DIFFUSION, HANGING DROP |
