1JJ7
Crystal Structure of the C-terminal ATPase domain of human TAP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 62 |
| Unit cell lengths | 87.570, 87.570, 79.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b0u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.053 | 0.401 |
| Number of reflections | 13755 | 1364 * |
| <I/σ(I)> | 15.9 | 3.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | 20% PEG8000, 100 mM sodium cacodylate, 200 mM magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | |
| 10 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | ATP | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | drop | protein | 10 (mg/ml) | |
| 8 | 1 | reservoir | PEG8000 | 20 (%) | |
| 9 | 1 | reservoir | sodium cacodylate | 100 (mM) |
