1JJ3

CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN AT PH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	27.415, 62.803, 60.935
Unit cell angles	90.00, 92.72, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.176
Rwork	0.176
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.005
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.077 *	0.216 *
Total number of observations	68752 *
Number of reflections	19151	1889 *
<I/σ(I)>	9.3
Completeness [%]	99.5	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Na-acetate buffer, pH 4.6, 10% NaCl, LIQUID DIFFUSION at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	acetate	0.04 (M)	pH4.6